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Propolis is a natural resinous substance collected by honeybees from buds and exu-dates of trees. The material has attracted much attention in recent years as a functional food component since it possesses various biological properties, including antimicro-bial, antioxidative, and anti-ulcer properties. In this study, the performance of accel-erated solvent extraction (ASE) was assessed and compared with varying methods of extraction: soaking (maceration), ultrasonication, and microwave-assisted methods. Gas chromatography-mass spectrometry (GC-MS) and other spectroscopic tech-niques, such as absorbance and fluorescence, were employed to assess the efficiency in the extraction of natural products. The antioxidant activity and phenolic content of the different extracts were also determined. Results showed samples obtained from the microwave method showed the highest yield in the extraction of bioactive com-pounds. Although microwave showed the best method in this study, some issues and recommendations on ASE application for extracting natural products from bee prop-olis were discussed. Given the ease in controlling extraction temperature with ASE, this technique has a great potential to be a better method for extraction of heat-labile natural products from propolis should optimization of conditions for extraction were further performed.